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control shrna lentiviral particles a  (Santa Cruz Biotechnology)
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Santa Cruz Biotechnology control shrna lentiviral particles a
Effect of Pz modulation on ASM proliferation and Wnt signaling. The effect of Pz1 agonist (Yoda1) and antagonist (GsMTx4) on ASM cell proliferation was evaluated using the CyQuant assay in all 4 groups (A). Baseline cell proliferation for young non-asthmatic ASM is indicated by the blue dotted line and used for comparison. Cells were treated with vehicle, Yoda1 (10 µm), and GsMTx4 (1 µm). No consistent pattern of drug, age, disease, or Pz modulation was noted on ASM cell proliferation. Expression of canonical Wnt (Wnt3a) (B), and non-canonical Wnt5ab (C) β-catenin (D) and GSK3β (E) elements was determined in cells where Pz1 channel expression was suppressed by <t>shRNA.</t> ASM cells failed to show the expected increase in these elements, even when exposed to mechanical stretch (representative electropherograms are shown in (F). Cells were verified for knockdown efficiency by using standard Western blot analysis (G) ** P < .01 vs negative shRNA. N = 5-6 patients per group, mean ± SEM for box plots.
Control Shrna Lentiviral Particles A, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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control shrna lentiviral particles a - by Bioz Stars, 2026-10
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addgene addgene plasmid  (Addgene inc)
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Effect of Pz modulation on ASM proliferation and Wnt signaling. The effect of Pz1 agonist (Yoda1) and antagonist (GsMTx4) on ASM cell proliferation was evaluated using the CyQuant assay in all 4 groups (A). Baseline cell proliferation for young non-asthmatic ASM is indicated by the blue dotted line and used for comparison. Cells were treated with vehicle, Yoda1 (10 µm), and GsMTx4 (1 µm). No consistent pattern of drug, age, disease, or Pz modulation was noted on ASM cell proliferation. Expression of canonical Wnt (Wnt3a) (B), and non-canonical Wnt5ab (C) β-catenin (D) and GSK3β (E) elements was determined in cells where Pz1 channel expression was suppressed by <t>shRNA.</t> ASM cells failed to show the expected increase in these elements, even when exposed to mechanical stretch (representative electropherograms are shown in (F). Cells were verified for knockdown efficiency by using standard Western blot analysis (G) ** P < .01 vs negative shRNA. N = 5-6 patients per group, mean ± SEM for box plots.
Addgene Addgene Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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addgene addgene plasmid - by Bioz Stars, 2026-10
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non targeting scrambled sirna  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology non targeting scrambled sirna
Effect of Pz modulation on ASM proliferation and Wnt signaling. The effect of Pz1 agonist (Yoda1) and antagonist (GsMTx4) on ASM cell proliferation was evaluated using the CyQuant assay in all 4 groups (A). Baseline cell proliferation for young non-asthmatic ASM is indicated by the blue dotted line and used for comparison. Cells were treated with vehicle, Yoda1 (10 µm), and GsMTx4 (1 µm). No consistent pattern of drug, age, disease, or Pz modulation was noted on ASM cell proliferation. Expression of canonical Wnt (Wnt3a) (B), and non-canonical Wnt5ab (C) β-catenin (D) and GSK3β (E) elements was determined in cells where Pz1 channel expression was suppressed by <t>shRNA.</t> ASM cells failed to show the expected increase in these elements, even when exposed to mechanical stretch (representative electropherograms are shown in (F). Cells were verified for knockdown efficiency by using standard Western blot analysis (G) ** P < .01 vs negative shRNA. N = 5-6 patients per group, mean ± SEM for box plots.
Non Targeting Scrambled Sirna, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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non targeting scrambled sirna - by Bioz Stars, 2026-10
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control sirna 116  (Santa Cruz Biotechnology)
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Santa Cruz Biotechnology control sirna 116
Effect of Pz modulation on ASM proliferation and Wnt signaling. The effect of Pz1 agonist (Yoda1) and antagonist (GsMTx4) on ASM cell proliferation was evaluated using the CyQuant assay in all 4 groups (A). Baseline cell proliferation for young non-asthmatic ASM is indicated by the blue dotted line and used for comparison. Cells were treated with vehicle, Yoda1 (10 µm), and GsMTx4 (1 µm). No consistent pattern of drug, age, disease, or Pz modulation was noted on ASM cell proliferation. Expression of canonical Wnt (Wnt3a) (B), and non-canonical Wnt5ab (C) β-catenin (D) and GSK3β (E) elements was determined in cells where Pz1 channel expression was suppressed by <t>shRNA.</t> ASM cells failed to show the expected increase in these elements, even when exposed to mechanical stretch (representative electropherograms are shown in (F). Cells were verified for knockdown efficiency by using standard Western blot analysis (G) ** P < .01 vs negative shRNA. N = 5-6 patients per group, mean ± SEM for box plots.
Control Sirna 116, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/control+shrna/LRRK2+siRNA/10__1016_slash_j__jpet__2026__105035-60-4-7
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lentiviral non targeting control shrna  (Genechem)
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Genechem lentiviral non targeting control shrna
Effect of Pz modulation on ASM proliferation and Wnt signaling. The effect of Pz1 agonist (Yoda1) and antagonist (GsMTx4) on ASM cell proliferation was evaluated using the CyQuant assay in all 4 groups (A). Baseline cell proliferation for young non-asthmatic ASM is indicated by the blue dotted line and used for comparison. Cells were treated with vehicle, Yoda1 (10 µm), and GsMTx4 (1 µm). No consistent pattern of drug, age, disease, or Pz modulation was noted on ASM cell proliferation. Expression of canonical Wnt (Wnt3a) (B), and non-canonical Wnt5ab (C) β-catenin (D) and GSK3β (E) elements was determined in cells where Pz1 channel expression was suppressed by <t>shRNA.</t> ASM cells failed to show the expected increase in these elements, even when exposed to mechanical stretch (representative electropherograms are shown in (F). Cells were verified for knockdown efficiency by using standard Western blot analysis (G) ** P < .01 vs negative shRNA. N = 5-6 patients per group, mean ± SEM for box plots.
Lentiviral Non Targeting Control Shrna, supplied by Genechem, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/control+shrna/control+non+targeting/pmc13276326-12-0-5
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lentiviral non targeting control shrna - by Bioz Stars, 2026-10
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scrambled shrna plasmid transfection  (Santa Cruz Biotechnology)
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Santa Cruz Biotechnology scrambled shrna plasmid transfection
Effect of Pz modulation on ASM proliferation and Wnt signaling. The effect of Pz1 agonist (Yoda1) and antagonist (GsMTx4) on ASM cell proliferation was evaluated using the CyQuant assay in all 4 groups (A). Baseline cell proliferation for young non-asthmatic ASM is indicated by the blue dotted line and used for comparison. Cells were treated with vehicle, Yoda1 (10 µm), and GsMTx4 (1 µm). No consistent pattern of drug, age, disease, or Pz modulation was noted on ASM cell proliferation. Expression of canonical Wnt (Wnt3a) (B), and non-canonical Wnt5ab (C) β-catenin (D) and GSK3β (E) elements was determined in cells where Pz1 channel expression was suppressed by <t>shRNA.</t> ASM cells failed to show the expected increase in these elements, even when exposed to mechanical stretch (representative electropherograms are shown in (F). Cells were verified for knockdown efficiency by using standard Western blot analysis (G) ** P < .01 vs negative shRNA. N = 5-6 patients per group, mean ± SEM for box plots.
Scrambled Shrna Plasmid Transfection, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/control+shrna/Control+shRNA+Plasmid-A/pm41957523-268-0-4
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scrambled shrna plasmid transfection - by Bioz Stars, 2026-10
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sirnas  (MedChemExpress)
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MedChemExpress sirnas
Effect of Pz modulation on ASM proliferation and Wnt signaling. The effect of Pz1 agonist (Yoda1) and antagonist (GsMTx4) on ASM cell proliferation was evaluated using the CyQuant assay in all 4 groups (A). Baseline cell proliferation for young non-asthmatic ASM is indicated by the blue dotted line and used for comparison. Cells were treated with vehicle, Yoda1 (10 µm), and GsMTx4 (1 µm). No consistent pattern of drug, age, disease, or Pz modulation was noted on ASM cell proliferation. Expression of canonical Wnt (Wnt3a) (B), and non-canonical Wnt5ab (C) β-catenin (D) and GSK3β (E) elements was determined in cells where Pz1 channel expression was suppressed by <t>shRNA.</t> ASM cells failed to show the expected increase in these elements, even when exposed to mechanical stretch (representative electropherograms are shown in (F). Cells were verified for knockdown efficiency by using standard Western blot analysis (G) ** P < .01 vs negative shRNA. N = 5-6 patients per group, mean ± SEM for box plots.
Sirnas, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/control+shrna/SiRNA+Negative+Control/pmc13221111-113-7-13
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negative control sirna  (MedChemExpress)
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MedChemExpress negative control sirna
Effect of Pz modulation on ASM proliferation and Wnt signaling. The effect of Pz1 agonist (Yoda1) and antagonist (GsMTx4) on ASM cell proliferation was evaluated using the CyQuant assay in all 4 groups (A). Baseline cell proliferation for young non-asthmatic ASM is indicated by the blue dotted line and used for comparison. Cells were treated with vehicle, Yoda1 (10 µm), and GsMTx4 (1 µm). No consistent pattern of drug, age, disease, or Pz modulation was noted on ASM cell proliferation. Expression of canonical Wnt (Wnt3a) (B), and non-canonical Wnt5ab (C) β-catenin (D) and GSK3β (E) elements was determined in cells where Pz1 channel expression was suppressed by <t>shRNA.</t> ASM cells failed to show the expected increase in these elements, even when exposed to mechanical stretch (representative electropherograms are shown in (F). Cells were verified for knockdown efficiency by using standard Western blot analysis (G) ** P < .01 vs negative shRNA. N = 5-6 patients per group, mean ± SEM for box plots.
Negative Control Sirna, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/control+shrna/SiRNA+Negative+Control/pm42346098-92-13-21
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hy  (MedChemExpress)
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Effect of Pz modulation on ASM proliferation and Wnt signaling. The effect of Pz1 agonist (Yoda1) and antagonist (GsMTx4) on ASM cell proliferation was evaluated using the CyQuant assay in all 4 groups (A). Baseline cell proliferation for young non-asthmatic ASM is indicated by the blue dotted line and used for comparison. Cells were treated with vehicle, Yoda1 (10 µm), and GsMTx4 (1 µm). No consistent pattern of drug, age, disease, or Pz modulation was noted on ASM cell proliferation. Expression of canonical Wnt (Wnt3a) (B), and non-canonical Wnt5ab (C) β-catenin (D) and GSK3β (E) elements was determined in cells where Pz1 channel expression was suppressed by <t>shRNA.</t> ASM cells failed to show the expected increase in these elements, even when exposed to mechanical stretch (representative electropherograms are shown in (F). Cells were verified for knockdown efficiency by using standard Western blot analysis (G) ** P < .01 vs negative shRNA. N = 5-6 patients per group, mean ± SEM for box plots.
Hy, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/control+shrna/SiRNA+Negative+Control/pmc13264372-85-6-3
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negative control shrna nc shrna  (Genechem)
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Genechem negative control shrna nc shrna
Effect of Pz modulation on ASM proliferation and Wnt signaling. The effect of Pz1 agonist (Yoda1) and antagonist (GsMTx4) on ASM cell proliferation was evaluated using the CyQuant assay in all 4 groups (A). Baseline cell proliferation for young non-asthmatic ASM is indicated by the blue dotted line and used for comparison. Cells were treated with vehicle, Yoda1 (10 µm), and GsMTx4 (1 µm). No consistent pattern of drug, age, disease, or Pz modulation was noted on ASM cell proliferation. Expression of canonical Wnt (Wnt3a) (B), and non-canonical Wnt5ab (C) β-catenin (D) and GSK3β (E) elements was determined in cells where Pz1 channel expression was suppressed by <t>shRNA.</t> ASM cells failed to show the expected increase in these elements, even when exposed to mechanical stretch (representative electropherograms are shown in (F). Cells were verified for knockdown efficiency by using standard Western blot analysis (G) ** P < .01 vs negative shRNA. N = 5-6 patients per group, mean ± SEM for box plots.
Negative Control Shrna Nc Shrna, supplied by Genechem, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/control+shrna/control+negative/pm42298489-82-8-15
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Effect of Pz modulation on ASM proliferation and Wnt signaling. The effect of Pz1 agonist (Yoda1) and antagonist (GsMTx4) on ASM cell proliferation was evaluated using the CyQuant assay in all 4 groups (A). Baseline cell proliferation for young non-asthmatic ASM is indicated by the blue dotted line and used for comparison. Cells were treated with vehicle, Yoda1 (10 µm), and GsMTx4 (1 µm). No consistent pattern of drug, age, disease, or Pz modulation was noted on ASM cell proliferation. Expression of canonical Wnt (Wnt3a) (B), and non-canonical Wnt5ab (C) β-catenin (D) and GSK3β (E) elements was determined in cells where Pz1 channel expression was suppressed by shRNA. ASM cells failed to show the expected increase in these elements, even when exposed to mechanical stretch (representative electropherograms are shown in (F). Cells were verified for knockdown efficiency by using standard Western blot analysis (G) ** P < .01 vs negative shRNA. N = 5-6 patients per group, mean ± SEM for box plots.

Journal: American Journal of Respiratory Cell and Molecular Biology

Article Title: Piezo1 mediates stretch-induced Wnt/β-catenin signaling and extracellular matrix remodeling in aging asthmatic airway smooth muscle

doi: 10.1093/ajrcmb/aanag058

Figure Lengend Snippet: Effect of Pz modulation on ASM proliferation and Wnt signaling. The effect of Pz1 agonist (Yoda1) and antagonist (GsMTx4) on ASM cell proliferation was evaluated using the CyQuant assay in all 4 groups (A). Baseline cell proliferation for young non-asthmatic ASM is indicated by the blue dotted line and used for comparison. Cells were treated with vehicle, Yoda1 (10 µm), and GsMTx4 (1 µm). No consistent pattern of drug, age, disease, or Pz modulation was noted on ASM cell proliferation. Expression of canonical Wnt (Wnt3a) (B), and non-canonical Wnt5ab (C) β-catenin (D) and GSK3β (E) elements was determined in cells where Pz1 channel expression was suppressed by shRNA. ASM cells failed to show the expected increase in these elements, even when exposed to mechanical stretch (representative electropherograms are shown in (F). Cells were verified for knockdown efficiency by using standard Western blot analysis (G) ** P < .01 vs negative shRNA. N = 5-6 patients per group, mean ± SEM for box plots.

Article Snippet: Control shRNA lentiviral particles-A (sc-108080) and Pz1 shRNA lentiviral particles (sc-93227-V) were procured from Santa Cruz Biotechnology (TX, USA).

Techniques: CyQUANT Assay, Comparison, Expressing, shRNA, Knockdown, Western Blot, Analysis